Anti-Ajuba antibody (ab64451)
Key features and details
- Rabbit polyclonal to Ajuba
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ajuba antibody
See all Ajuba primary antibodies -
Description
Rabbit polyclonal to Ajuba -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Ajuba aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93791) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HepG2; MEL-1; WI38.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Target
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Relevance
Ajuba is a member of LIM domain containing proteins that contribute to cell fate determination and regulate cell proliferation and differentiation. Ajuba is also involved in the regulation of actin cytoskeleton dynamics and cell migration. Ajuba plays a important role in the regulation of the kinase activity of AURKA/Aurora A for mitotic commitment. Is also a component of the IL 1 signaling pathway modulating IL 1 induced NF kappa B activation by influencing the assembly and activity of the PRKCZ/SQSTM1/TRAF6 multiprotein signaling complex. Ajuba is also a negative regulator of the Wnt signaling pathway. -
Cellular localization
Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Centrosome. Note: Shuttles between the cytoplasm and the nucleus. -
Database links
- Entrez Gene: 84962 Human
- Entrez Gene: 16475 Mouse
- Entrez Gene: 85265 Rat
- Omim: 609066 Human
- SwissProt: Q96IF1 Human
- SwissProt: Q91XC0 Mouse
- SwissProt: Q5U2Z2 Rat
- Unigene: 655832 Human
see all -
Alternative names
- Ajuba LIM protein antibody
- Ajuba, mouse, homolog of antibody
- Jub ajuba homolog antibody
see all
Images
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Western blot - Anti-Ajuba antibody (ab64451)All lanes : Anti-Ajuba antibody (ab64451) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 3 :WI-38 whole cell lysate (ab3960)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 23 kDa, 48 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Datasheets and documents
References (2)
ab64451 has been referenced in 2 publications.
- Fowler S et al. LIM Protein Ajuba associates with the RPA complex through direct cell cycle-dependent interaction with the RPA70 subunit. Sci Rep 8:9536 (2018). PubMed: 29934626
- Kalan S et al. LIM Protein Ajuba Participates in the Repression of the ATR-Mediated DNA Damage Response. Front Genet 4:95 (2013). Human . PubMed: 23755068
Images
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Western blot - Anti-Ajuba antibody (ab64451)All lanes : Anti-Ajuba antibody (ab64451) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 3 :WI-38 whole cell lysate (ab3960)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 23 kDa, 48 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
