Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µg 264 681 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Product name

Anti-Ajuba antibody
See all Ajuba primary antibodies
Description

Rabbit polyclonal to Ajuba
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human Ajuba aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as ab93791)
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HepG2; MEL-1; WI38.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration
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Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab64451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
WB	Human
All applications	Mouse Rat

Application	Abreviews	Notes
WB		Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Relevance

Ajuba is a member of LIM domain containing proteins that contribute to cell fate determination and regulate cell proliferation and differentiation. Ajuba is also involved in the regulation of actin cytoskeleton dynamics and cell migration. Ajuba plays a important role in the regulation of the kinase activity of AURKA/Aurora A for mitotic commitment. Is also a component of the IL 1 signaling pathway modulating IL 1 induced NF kappa B activation by influencing the assembly and activity of the PRKCZ/SQSTM1/TRAF6 multiprotein signaling complex. Ajuba is also a negative regulator of the Wnt signaling pathway.
Cellular localization

Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Centrosome. Note: Shuttles between the cytoplasm and the nucleus.
Database links
- Entrez Gene: 84962 Human
- Entrez Gene: 16475 Mouse
- Entrez Gene: 85265 Rat
- Omim: 609066 Human
- SwissProt: Q96IF1 Human
- SwissProt: Q91XC0 Mouse
- SwissProt: Q5U2Z2 Rat
- Unigene: 655832 Human
- Unigene: 100253 Mouse
- Unigene: 144617 Rat
see all
Alternative names
- Ajuba LIM protein antibody
- Ajuba, mouse, homolog of antibody
- Jub ajuba homolog antibody
- Jub antibody
- LIM domain containing protein ajuba antibody
- MGC15563 antibody
- Protein ajuba antibody
see all

Publishing research using ab64451? Please let us know so that we can cite the reference in this datasheet.

ab64451 has been referenced in 2 publications.

Fowler S et al. LIM Protein Ajuba associates with the RPA complex through direct cell cycle-dependent interaction with the RPA70 subunit. Sci Rep 8:9536 (2018). PubMed: 29934626
Kalan S et al. LIM Protein Ajuba Participates in the Repression of the ATR-Mediated DNA Damage Response. Front Genet 4:95 (2013). Human . PubMed: 23755068