Anti-AIF antibody (ab137725)
Key features and details
- Rabbit polyclonal to AIF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AIF antibody
See all AIF primary antibodies -
Description
Rabbit polyclonal to AIF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 82-330 of Human AIF.
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Positive control
- H1299, HepG2 and Mouse liver whole cell lysates; SW480 xenograft tissue; HeLa cells; 293T, A431, Molt4 and Raji
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-AIF antibody (ab137725) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 67 kDa
7.5% SDS PAGE -
Anti-AIF antibody (ab137725) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 67 kDa
7.5% SDS PAGE -
Immunofluorescenct analysis of methanol-fixed HeLa cells labelling AIF with ab137725 at 1/200 dilution. Lower panel shows costaining with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded SW480 xenograft tissue labelling AIF with ab137725 at 1/100 dilution.