Anti-AHNAK antibody (ab135137)
Key features and details
- Rabbit polyclonal to AHNAK
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AHNAK antibody
See all AHNAK primary antibodies -
Description
Rabbit polyclonal to AHNAK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide corresponding to Human AHNAK aa 100-200 conjugated to keyhole limpet haemocyanin.
Database link: Q09666 -
Positive control
- This antibody gave a positive signal in JEG3 whole cell lysates within IF/ICC.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanAll applications MouseRatChimpanzeeGorillaOrangutanApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.AP-0016540
Notes ICC/IF
Use a concentration of 5 µg/ml.AP-0016540
Target
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Function
May be required for neuronal cell differentiation. -
Sequence similarities
Contains 1 PDZ (DHR) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79026 Human
- Entrez Gene: 66395 Mouse
- Entrez Gene: 191572 Rat
- Omim: 103390 Human
- SwissProt: Q09666 Human
- Unigene: 502756 Human
- Unigene: 203866 Mouse
- Unigene: 226586 Rat
see all -
Alternative names
- AHNAK antibody
- AHNAK nucleoprotein (desmoyokin) antibody
- AHNAK nucleoprotein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AHNAK antibody (ab135137)
ICC/IF image of ab135137 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab135137, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The expression profile observed is consistent with what has been described in the literature (PMID:7698224, PMID:7769263 and PMID:11535620).
Protocols
Datasheets and documents
References (0)
ab135137 has not yet been referenced specifically in any publications.
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AHNAK antibody (ab135137)
ICC/IF image of ab135137 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab135137, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The expression profile observed is consistent with what has been described in the literature (PMID:7698224, PMID:7769263 and PMID:11535620).
