Anti-AE2 antibody (ab42687)
Key features and details
- Rabbit polyclonal to AE2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AE2 antibody
See all AE2 primary antibodies -
Description
Rabbit polyclonal to AE2This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide corresponding to Human AE2 aa 200-300.
(Peptide available asab42686)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanAll applications MouseRatRabbitHorseGuinea pigCowOrangutanApplication Abreviews Notes ICC/IF Use at an assay dependent concentration.Notes ICC/IF
Use at an assay dependent concentration.Target
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Function
Plasma membrane anion exchange protein of wide distribution. -
Sequence similarities
Belongs to the anion exchanger (TC 2.A.31) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 404084 Cow
- Entrez Gene: 791243 Horse
- Entrez Gene: 6522 Human
- Entrez Gene: 20535 Mouse
- Entrez Gene: 100172030 Orangutan
- Entrez Gene: 100009159 Rabbit
- Entrez Gene: 24780 Rat
- Omim: 109280 Human
see all -
Alternative names
- AE 2 antibody
- AE2 anion exchanger antibody
- Anion exchange protein 2 antibody
see all
Images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.-
Immunocytochemistry/ Immunofluorescence - Anti-AE2 antibody (ab42687)ICC/IF image of ab42687 stained MCF7 cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42687, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
Protocols
Datasheets and documents
References (5)
ab42687 has been referenced in 5 publications.
- Hwang S et al. Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5. J Enzyme Inhib Med Chem 34:179-188 (2019). PubMed: 30451023
- Hwang S et al. Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration. Molecules 24:N/A (2019). PubMed: 31546841
- Yin K et al. SLC26A Gene Family Participate in pH Regulation during Enamel Maturation. PLoS One 10:e0144703 (2015). IHC-P ; Rat . PubMed: 26671068
- Huang J et al. Basolateral chloride loading by the anion exchanger type 2: role in fluid secretion by the human airway epithelial cell line Calu-3. J Physiol 590:5299-316 (2012). PubMed: 22802585
- Kao L et al. Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene). J Biol Chem 286:32563-74 (2011). IHC-FoFr ; Mouse . PubMed: 21705333
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AE2 antibody (ab42687)ICC/IF image of ab42687 stained MCF7 cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42687, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
