Anti-AE2 antibody (ab226829)
Key features and details
- Rabbit polyclonal to AE2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AE2 antibody
See all AE2 primary antibodies -
Description
Rabbit polyclonal to AE2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human AE2 aa 1-50. The exact sequence is proprietary. (NP_001186621.1).
Database link: P04920 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226829 was affinity purified using an epitope specific to AE2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody (ab226829)
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for AE2 using ab226829 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
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Western blot - Anti-AE2 antibody (ab226829)All lanes : Anti-AE2 antibody (ab226829) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 137 kDa
Exposure time: 30 secondsLysates were prepared using RIPA lysis buffer.
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Immunoprecipitation - Anti-AE2 antibody (ab226829)AE2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using RIPA lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226829 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226829 at 1 µg/ml.
Lane 1: ab226829 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
