Anti-ADAMTS12 antibody (ab45041)
Key features and details
- Rabbit polyclonal to ADAMTS12
- Suitable for: WB
- Reacts with: Mouse, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-ADAMTS12 antibody
See all ADAMTS12 primary antibodies -
Description
Rabbit polyclonal to ADAMTS12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB African green monkey
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Immunogen
Synthetic peptide based on the propeptide region of Human ADAMTS12.
Read Abcam's proprietary immunogen policy (Peptide available as ab45237.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ADAMTS12 antibody (ab45041)All lanes : Anti-ADAMTS12 antibody (ab45041) at 1/1000 dilution
Lane 1 : Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with TPA)
Lane 2 : Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with IL1-alpha)
Lane 3 : Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with TNF-alpha)
Predicted band size: 178 kDa
Full-length ADAMTS12 has been detected at 178 kDa by WB. However, study looking at ADAM-TS 12 expression in COS-7 cells (Gal et al. (2001) J. Biol. Chem. 276: 17932), has detected an 83 kD COOH-terminal cleavage product.It is possible that the post-translation processing in COS-7 cells is different than that of other cells, that the epitope tag is cleaved during processing, or that the levels of the 83 kD product in other cells were below detection.
