Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (ab273515)
![Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (ab273515)](https://www.abcam.com/ps/products/273/ab273515/Images/ab273515-2-anti-adam10-antibody-epr2349174-immunocytochemistry-hct-116.jpg "Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (ab273515)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23491-74] to ADAM10 - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free
See all ADAM10 primary antibodies -
Description
Rabbit monoclonal [EPR23491-74] to ADAM10 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: LNCaP and HCT116 cells. Flow Cyt: HCT116 cells.
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General notes
ab273515 is the carrier-free version of ab252234. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23491-74 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (ab273515)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 cells labelling ADAM10 with ab252234 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous and weak cytoplasmic staining in HCT 116 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Low expression control: HeLa (PMID: 23091066).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252234).
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![Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (ab273515)](https://www.abcam.com/ps/products/273/ab273515/Images/ab273515-1-anti-adam10-antibody-epr2349174-immunocytochemistry-lncap.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (ab273515)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP cells labelling ADAM10 with ab252234 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous and weak cytoplasmic staining in LNCaP cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252234).
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![Flow Cytometry - Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (ab273515)](https://www.abcam.com/ps/products/273/ab273515/Images/ab273515-3-anti-adam10-antibody-epr2349174-flow-cytometry-hela.jpg)
Flow Cytometry - Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (ab273515)Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) (Left panel) / HCT 116 (Human colorectal carcinoma epithelial cell) (Right panel) cells labelling ADAM10 with ab252234 at 1/400 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Low expression control: HeLa (PMID: 23091066).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252234).
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![Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (ab273515)](https://www.abcam.com/ps/products/273/ab273515/Images/ab273515-4-benefits-of-recombinant-antibodies.png)
Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (ab273515)