Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Actin antibody [IGX3831H] (ab213251)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [IGX3831H] to Actin
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Actin antibody [IGX3831H]
    See all Actin primary antibodies

  • Description

    Human monoclonal [IGX3831H] to Actin

  • Host species

    Human

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Actin aa 150-400. The exact sequence is proprietary.
    Database link: P68133

  • Positive control

    • ICC/IF: HeLa, NIH3T3 cells WB: Human and mouse colon, HeLa, Jurkat, NIH3T3, PANC1, C2C12 whole cell lysates and human skeletal muscle.

  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody.
    Human monoclonal antibody.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)
    Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)

    Ab213251 staining Actin in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213251 at a 5µg/ml concentration, then detected with a goat anti-human (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Actin antibody [IGX3831H] (ab213251)
    Western blot - Anti-Actin antibody [IGX3831H] (ab213251)
    All lanes : Anti-Actin [IGX3831H] at 1 µg/ml

    Lane 1 : Human colon tissue lysate
    Lane 2 : Mouse colon tissue lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : Jurkat whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate
    Lane 6 : PANC1 whole cell lysate
    Lane 7 : C2C12 whole cell lysate
    Lane 8 : Human skeletal muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 42 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes.  The membrane was then blocked for an hour using 3% milk before being incubated with ab213251 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)
    Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)

    Ab213251 staining Actin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213251 at a 5µg/ml concentration, then detected with a goat anti-human (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-Actin antibody [IGX3831H] (ab213251)
    Anti-Actin antibody [IGX3831H] (ab213251)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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