Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸ 10 µl 67 008 ₸

Order now and get it on Thursday March 04, 2021

Anti-Actin antibody [IGX3831H] (ab213251)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Human monoclonal [IGX3831H] to Actin
Suitable for: WB, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-Actin antibody [IGX3831H]
See all Actin primary antibodies
Description

Human monoclonal [IGX3831H] to Actin
Host species

Human

Tested Applications & Species

Application	Species
ICC/IF	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment within Human Actin aa 150-400. The exact sequence is proprietary.
Database link: P68133
Positive control
- ICC/IF: HeLa, NIH3T3 cells WB: Human and mouse colon, HeLa, Jurkat, NIH3T3, PANC1, C2C12 whole cell lysates and human skeletal muscle.
General notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant antibody.
Human monoclonal antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

IGX3831H
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)

Ab213251 staining Actin in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213251 at a 5µg/ml concentration, then detected with a goat anti-human (Alexa Fluor^® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Anti-Actin antibody [IGX3831H] (ab213251)

All lanes : Anti-Actin [IGX3831H] at 1 µg/ml

Lane 1 : Human colon tissue lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lane 6 : PANC1 whole cell lysate
Lane 7 : C2C12 whole cell lysate
Lane 8 : Human skeletal muscle tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size: 42 kDa

Exposure time: 1 minute

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213251 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)

Ab213251 staining Actin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213251 at a 5µg/ml concentration, then detected with a goat anti-human (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-Actin antibody [IGX3831H] (ab213251)

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