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Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19038] to ACOX1/AOX - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free
    See all ACOX1/AOX primary antibodies

  • Description

    Rabbit monoclonal [EPR19038] to ACOX1/AOX - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: C6 cells.

  • General notes

    ab238939 is the carrier-free version of ab184032 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab238939 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as ACOX1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)
    Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling ACOX1/AOX with ab184032 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR19038] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184032 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184032).

  • Flow Cytometry - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)
    Flow Cytometry - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)

    Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast) cell line labeling ACOX1/AOX with ab184032 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR19038] - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184032).

  • Immunoprecipitation - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)
    Immunoprecipitation - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)

    ACOX1/AOX was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab184032 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184032 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: NIH/3T3 whole cell lysate 10µg (Input).
    Lane 2: ab184032 IP in NIH/3T3 whole cell lysate.
    Lane 3: Rabbit IgG, monoclonal [EPR19038] - Isotype Control (ab172730) instead of ab184032 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184032).

  • Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)
    Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling ACOX1/AOX with ab184032 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR19038] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184032 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184032).

     

  • Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)
    Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (ab238939)

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