Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8623] to ACBD3 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ACBD3 antibody [EPR8623] - BSA and Azide free
    See all ACBD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8623] to ACBD3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248750 is the carrier-free version of ab134952 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248750 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
    Western blot - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)

    This data was developed using ab134952, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: ACBD3 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab134952 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab134952 was shown to specifically react with ACBD3 when ACBD3 knockout samples were used. Wild-type and ACBD3 knockout samples were subjected to SDS-PAGE. ab134952 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
    Western blot - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
    All lanes : Anti-ACBD3 antibody [EPR8623] (ab134952) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 4 : LNCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 61 kDa



    This data was developed using ab134952, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
    Flow Cytometry - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
    This data was developed using ab134952, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized K562 cells labelling ACBD3 using ab134952 (red) at 1/100 dilution and rabbit IgG negative control (green).
  • OI-RD Scanning - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
    OI-RD Scanning - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
    This data was developed using ab134952, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
    Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)

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