Fructosamine Assay Kit (ab228558)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Serum
Overview
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Product name
Fructosamine Assay Kit -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Quantitative -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Fructosamine Assay Kit (ab228558) is a fast and convenient microplate-based colorimetric assay for the direct determination of fructosamine levels in serum. The assay is based on the ability of fructosamine to reduce nitroblue tetrazolium (NBT), forming a colored end-product (purple) under alkaline conditions. The formation rate of formazan is proportional to the concentration of fructosamine in samples and the increase in absorbance (OD 530nm) can be monitored using a spectrophotometer.
The kit includes a thiol blocking reagent and a sample cleaning mix that minimizes the interference of other endogenous reducing agents which ensures accurate measurements of Fructosamine in biological samples.
The assay is simple, reproducible and can detect as low as 20 µmol/L of fructosamine in samples. Assay time ~ 1 hour.
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Notes
Fructosamines are stable glycated proteins that are formed by a non-enzymatic reaction between glucose and serum proteins (usually albumin). Elevated concentrations of fructosamine can be found in serum samples of diabetic patients and its detection can be used to assess the glycemic status of diabetics. The half-life of albumin is shorter when compared to hemoglobin (t1/2= 20 days vs. 50 days respectively). Thus, fructosamine levels reflect the efficacy of treatment in diabetic patients during short-term periods, and provide earlier and more sensitive detection for diabetes than many other carbohydrate tests.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Fructosamine Buffer A 1 x 25ml Fructosamine Buffer B 1 x 40ml Fructosamine Calibrator (3.2 mM) 1 x 180µl NBT (in DMF) 1 x 580µl Sample Cleaning Mix (Lyophilized) 1 vial Thiol Blocking Reagent 1 vial
Images
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Example Data
Fructosamine concentrations (µmol/L): Estimated Fructosamine concentrations are 222.4 ± 20.7 µmol/L in healthy human Serum (10 µl) and 458.8 ± 43.9 µmol/L in Human Diabetic Serum (10 µl), respectively. Assays were performed following the kit protocol.
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Example DataFructosamine reaction curves.
