Anti-ABCF2 antibody (ab86178)
Key features and details
- Rabbit polyclonal to ABCF2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ABCF2 antibody
See all ABCF2 primary antibodies -
Description
Rabbit polyclonal to ABCF2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide corresponding to a region between residue 1 and 50 of human ABCF2 using the numbering given in entry NP_009120.1.
-
Positive control
- HeLa whole cell lysate 293T whole cell lysate NIH3T3 whole cell lysate
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ABCF2 antibody (ab86178)All lanes : Anti-ABCF2 antibody (ab86178) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Observed band size: 71 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
-
Immunoprecipitation - Anti-ABCF2 antibody (ab86178)Detection of Human ABCF2 by Immunoprecipitation, using ab86178 at 3µg/mg lysate. Image shows immunoprecipitated ABCF2 detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with ab86178 at 1 µg/ml in lane 1, antibodies recognising a downstream eptiope in lanes 2 and 3, and control IgG in lane 4. Detection: Chemiluminescence with an exposure time of 10 seconds.
