Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5126(2)] to AAK1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free
    See all AAK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5126(2)] to AAK1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248763 is the carrier-free version of ab134971 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248763 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)
    Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)
    Anti-AAK1 antibody [EPR5126(2)] (ab134971) at 1/1000 dilution + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 20ug

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 104 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab134971, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)
    Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)
    Anti-AAK1 antibody [EPR5126(2)] (ab134971) at 1/10000 dilution + His-tagged human AAK1 recombinant protein (aa 1-365) 10ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 104 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    This data was developed using ab134971, the same antibody clone in a different buffer formulation.

  • Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)
    Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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