Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)
![Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)](https://www.abcam.com/ps/products/248/ab248763/Images/ab248763-447684-AntiAAK1antibodywesternblotab134971.jpg "Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5126(2)] to AAK1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free
See all AAK1 primary antibodies -
Description
Rabbit monoclonal [EPR5126(2)] to AAK1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248763 is the carrier-free version of ab134971 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248763 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5126(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)Anti-AAK1 antibody [EPR5126(2)] (ab134971) at 1/1000 dilution + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 20ug
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 104 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?This data was developed using ab134971, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)](https://www.abcam.com/ps/products/248/ab248763/Images/ab248763-447683-AntiAAK1antibodywesternblotab134971.jpg)
Western blot - Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)Anti-AAK1 antibody [EPR5126(2)] (ab134971) at 1/10000 dilution + His-tagged human AAK1 recombinant protein (aa 1-365) 10ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 104 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?This data was developed using ab134971, the same antibody clone in a different buffer formulation.
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![Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)](https://www.abcam.com/ps/products/248/ab248763/Images/ab248763-3-benefits-of-recombinant-antibodies.png)
Anti-AAK1 antibody [EPR5126(2)] - BSA and Azide free (ab248763)
