Anti-A2B5 antibody [105] (ab53521)
![Anti-A2B5 antibody [105] (ab53521)](https://www.abcam.com/ps/products/53/ab53521/Images/ab53521-4-anti-a2b5-105-immunocytochemistry-antibody-primary-neurons-div14-rat.jpg "Anti-A2B5 antibody [105] (ab53521)")
Key features and details
- Mouse monoclonal [105] to A2B5
- Suitable for: ICC, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgM
Overview
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Product name
Anti-A2B5 antibody [105] -
Description
Mouse monoclonal [105] to A2B5 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC MouseRat
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Immunogen
Tissue, cells or virus corresponding to Chicken A2B5.
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Positive control
- Type II astrocytes, human neural progenitors.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Purified IgM -
Clonality
Monoclonal -
Clone number
105 -
Myeloma
P3-x63-Ag8 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry - Anti-A2B5 antibody [105] (ab53521)
ab53521 staining A2B5 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab53521 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Immunocytochemistry - Anti-A2B5 antibody [105] (ab53521)](https://www.abcam.com/ps/products/53/ab53521/Images/ab53521-66404-anti-a2b5-antibody-105-immunofluorescence.jpg)
Immunocytochemistry - Anti-A2B5 antibody [105] (ab53521)ICC/IF image of ab53521 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53521, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). -
![Immunocytochemistry - Anti-A2B5 antibody [105] (ab53521)](https://www.abcam.com/ps/products/53/ab53521/Images/ab53521-66403-anti-a2b5-antibody-105-immunofluorescence.jpg)
Immunocytochemistry - Anti-A2B5 antibody [105] (ab53521) This image is courtesy of an Abreview submitted by Mr Carl HobbsA2B5 antibody [105] - Stem Cell Marker (ab53521) used in immunocytochemical detection on Cor1 (mouse) neuronal stem cells. Cor1 neuronal stem cells were fixed in formaldehyde, permeabilized, blocked in 1% BSA for 30 mins at RT. ab53521 was incubated at 1/500 for 16 hours in TBS/BSA/azide/0.5% Triton. Secondary Antibody:anti mouse IgM conjugated: to Alexa Fluor® 488 (1/1000).
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![Flow Cytometry - Anti-A2B5 antibody [105] (ab53521)](https://www.abcam.com/ps/products/53/ab53521/Images/ab53521-66402-anti-a2b5-antibody-105-flow-cytometry.jpg)
Flow Cytometry - Anti-A2B5 antibody [105] (ab53521)Overlay histogram showing SH-SY5Y cells stained with ab53521 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53521, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
