Alexa Fluor® 594 Anti-BRG1 antibody [EPNCIR111A] (ab207052)
![Alexa Fluor® 594 Anti-BRG1 antibody [EPNCIR111A] (ab207052)](https://www.abcam.com/ps/products/207/ab207052/Images/ab207052-414960-AntiBRG1antibodyImmunocytochemistry.jpg "Alexa Fluor® 594 Anti-BRG1 antibody [EPNCIR111A] (ab207052)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 594 Rabbit monoclonal [EPNCIR111A] to BRG1
- Suitable for: ICC
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Overview
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Product name
Alexa Fluor® 594 Anti-BRG1 antibody [EPNCIR111A]
See all BRG1 primary antibodies -
Description
Alexa Fluor® 594 Rabbit monoclonal [EPNCIR111A] to BRG1 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: SW480 and MCF7 cells
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General notes
This conjugated antibody has been KO validated based on the results obtained with the unconjugated clone: Anti-BRG1 antibody [EPNCIR111A] (ab110641).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR111A -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Alexa Fluor® 594 Anti-BRG1 antibody [EPNCIR111A] (ab207052)
ab207052 staining BRG1 in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207052 at a 1/50 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Immunocytochemistry - Alexa Fluor® 594 Anti-BRG1 antibody [EPNCIR111A] (ab207052)](https://www.abcam.com/ps/products/207/ab207052/Images/ab207052-255588-anti-brg1-antibody-epncir111a-alexa-fluor-594-immunofluorescence.jpg)
Immunocytochemistry - Alexa Fluor® 594 Anti-BRG1 antibody [EPNCIR111A] (ab207052)ab207052 staining BRG1 in SW480 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207052 at a 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in SW480 cells fixed with 100% methanol (5 min)
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![Alexa Fluor® 594 Anti-BRG1 antibody [EPNCIR111A] (ab207052)](https://www.abcam.com/ps/products/207/ab207052/Images/ab207052-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 594 Anti-BRG1 antibody [EPNCIR111A] (ab207052)
