Anti-7-Ketocholesterol antibody [7E1] (ab243071)
![Anti-7-Ketocholesterol antibody [7E1] (ab243071)](https://www.abcam.com/ps/products/243/ab243071/Images/ab243071-332313-71.png "Anti-7-Ketocholesterol antibody [7E1] (ab243071)")
Key features and details
- Mouse monoclonal [7E1] to 7-Ketocholesterol
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG2b
Overview
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Product name
Anti-7-Ketocholesterol antibody [7E1] -
Description
Mouse monoclonal [7E1] to 7-Ketocholesterol -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 7-Ketocholesterol conjugated to keyhole limpet haemocyanin.
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Positive control
- WB: 7-ketocholesterol-BSA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
7E1 -
Isotype
IgG2b
Images
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Western blot - Anti-7-Ketocholesterol antibody [7E1] (ab243071)All lanes : Anti-7-Ketocholesterol antibody [7E1] (ab243071) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : BSA at 2 µg
Lane 3 : 7-ketocholesterol-BSA at 0.5 µg
Lane 4 : 7-ketocholesterol-BSA at 2 µg
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesPrimary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
