Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
![Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)](https://www.abcam.com/ps/products/247/ab247373/Images/ab247373-408232-anti-4-1bbl-antibody-epr1172y-bsa-and-azide-free-western-blot.jpg "Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1172Y] to 4-1BBL - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free
See all 4-1BBL primary antibodies -
Description
Rabbit monoclonal [EPR1172Y] to 4-1BBL - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247373 is the carrier-free version of ab68185 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247373 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1172Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + Raji whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 25,32 kDa why is the actual band size different from the predicted?This data was developed using ab68185, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)](https://www.abcam.com/ps/products/247/ab247373/Images/ab247373-405505-anti-4-1bbl-antibody-epr1172y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + HEK293 whole cell lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 25-32 kDa why is the actual band size different from the predicted?This data was developed using ab68185, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)](https://www.abcam.com/ps/products/247/ab247373/Images/ab247373-403555-anti-4-1bbl-antibody-epr1172y-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)This data was developed using ab68185, the same antibody clone in a different buffer formulation.
ab68185 (purified) at 1/50 immunoprecipitating 4-1BBL in HEK293 whole cell lysate.
Lane 1 (input): HEK293 whole cell lysate (10µg)
Lane 2 (+): ab68185 + HEK293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68185 in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)](https://www.abcam.com/ps/products/247/ab247373/Images/ab247373-397912-anti-4-1bbl-antibody-epr1172y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/2000 dilution (unpurified) + 293T cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?This data was developed using ab68185, the same antibody clone in a different buffer formulation.
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![Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)](https://www.abcam.com/ps/products/247/ab247373/Images/ab247373-5-benefits-of-recombinant-antibodies.png)
Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)