Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1172Y] to 4-1BBL - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free
    See all 4-1BBL primary antibodies

  • Description

    Rabbit monoclonal [EPR1172Y] to 4-1BBL - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247373 is the carrier-free version of ab68185 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247373 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
    Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
    Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + Raji whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 27 kDa
    Observed band size: 25,32 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab68185, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
    Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
    Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + HEK293 whole cell lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 27 kDa
    Observed band size: 25-32 kDa why is the actual band size different from the predicted?



    This data was developed using ab68185, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
    Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)

    This data was developed using ab68185, the same antibody clone in a different buffer formulation.

    ab68185 (purified) at 1/50 immunoprecipitating 4-1BBL in HEK293 whole cell lysate.

    Lane 1 (input): HEK293 whole cell lysate (10µg)

    Lane 2 (+): ab68185 + HEK293 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68185 in HEK293 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
    Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
    Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/2000 dilution (unpurified) + 293T cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 27 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    This data was developed using ab68185, the same antibody clone in a different buffer formulation.

     

  • Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
    Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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