Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (ab251530)
![Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (ab251530)](https://www.abcam.com/ps/products/251/ab251530/Images/ab251530-403551-anti-2-methyladenosine-m2a-antibody-epr-19851-59-bsa-and-azide-free-immunoprecipitation.jpg "Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (ab251530)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR -19851-59] to 2-methyladenosine (m2A) - BSA and Azide free
- Suitable for: ELISA, IP
Overview
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Product name
Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free
See all 2-methyladenosine (m2A) primary antibodies -
Description
Rabbit monoclonal [EPR -19851-59] to 2-methyladenosine (m2A) - BSA and Azide free -
Host species
Rabbit -
Specificity
Has been developed to discriminate between the modified base 2-methyladenosine (m2A) and the unmodified counterpart Adenosine (A).
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Tested applications
Suitable for: ELISA, IPmore details -
Species reactivity
Reacts with: Synthetic fragment -
Immunogen
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251530 is the carrier-free version of ab211495. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251530 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR -19851-59 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (ab251530)
This data was developed using ab211495, the same antibody clone in a different buffer formulation.
The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.
ab211495 (0.2 µg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 µl) for 1h at RT.
Unmodified/modified oligonucleotides (5 µM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.
After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.
ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.
Lane 1: Buffer only.
Lane 2: Modified oligonucleotide (5 µM), 5' Biotin-mN.mN.mN.mN.mN.[ m2A]*.mN.mN.mN.mN.mN 3'
Lane 3: Unmodified oligonucleotide (5 µM), 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'
N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protectionBlocking buffer: 5% NFDM/TBST
Dilution buffer: TBST/0.1% Triton X-100/1 mM EDTA.
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![ELISA - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (ab251530)](https://www.abcam.com/ps/products/251/ab251530/Images/ab251530-1-anti-2-methyladenosine-m2a-antibody-epr-19851-59-bsa-and-azide-free-elisa.jpg)
ELISA - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (ab251530)This data was developed using ab211495, the same antibody clone in a different buffer formulation.Biotinylated m2A (modified) and A (unmodified) oligonucleotides with the below sequence were coated onto wells of a 96 well plate. Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[ m2A]*.mN.mN.mN.mN.mN 3’ Unmodified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3’ N - equimolar mixture of (A/U/G/C)
m - 2’O methyl protection
* - phosphorothioate protection ELISA was performed on 1.0 µg/ml of antigen using ab211495 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution. -
![Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (ab251530)](https://www.abcam.com/ps/products/251/ab251530/Images/ab251530-3-benefits-of-recombinant-antibodies.png)
Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (ab251530)