Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5873(2)] to 14-3-3 sigma/SFN - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free
    See all 14-3-3 sigma/SFN primary antibodies

  • Description

    Rabbit monoclonal [EPR5873(2)] to 14-3-3 sigma/SFN - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251175 is the carrier-free version of ab193667 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251175 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as 14-3-3 sigma

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
    Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
    All lanes : Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution

    Lane 1 : Human skin lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HACAT cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab193667, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
    This data was developed using ab193667, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 14-3-3 sigma/SFN with ab193667 at 1/600 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
    Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
    Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution + Human 14-3-3 sigma/SFN recombinant protein with His tag at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?



    This data was developed using ab193667, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human full length 14-3-3 sigma/SFN recombinant protein is a house-made one with His-Tag.

  • Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
    Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)

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