alpha-Mannosidase Assay Kit (ab272519)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Lysate
Overview
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Product name
alpha-Mannosidase Assay Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Lysate -
Assay type
Quantitative -
Range
1 mU/ml - 250 mU/ml -
Product overview
alpha-Mannosidase Assay Kit (ab272519) is a nonradioactive, colorimetric alpha-Mannosidase assay is based on the cleavage of 4-nitrophenol from a synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
Fast and sensitive: Linear detection range (10 μL sample): 1 to 250 U/L for a 10 minute reaction.
Convenient and high-throughput: Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Standard (12.5 mM Nitrophenol) 1 x 1ml Stop Reagent 1 x 12ml Substrate Buffer 1 x 10ml
Images
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Alpha-Mannosidase Assay Kit Activity Titration Curve
Example of alpha-Mannosidase Assay Kit activity titration.
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Alpha-Mannosidase Assay Kit Standard CurveTypical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of 4-Nitrophenol standard curve.