Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture extracts, Saliva, Serum, Suspension cells, Tissue
Overview
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Product name
Alpha-Glucosidase Activity Assay Kit (Colorimetric)
See all alpha-glucosidase kits -
Detection method
Colorimetric -
Sample type
Saliva, Serum, Cell culture extracts, Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Range
0.1 mU/well - 10 mU/well -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
In Abcam's Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093), α-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm). This is an easy, quick and high-throughput capable kit that can measure 0.1-10 mU of α-glucosidase activity in a variety of samples.
Visit our FAQs page for tips and troubleshooting.
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Notes
α-Glucosidase breaks down α-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, α-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase.
Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to α-glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Alpha Glucosidase Assay Buffer WM 1 x 25ml Alpha Glucosidase Positive Control 1 vial Alpha Glucosidase Substrate Mix Amber 1 x 0.3ml p-Nitrophenol Standard (100 mM) 1 x 100µl -
Research areas
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Relevance
a-Glucosidase breaks down a-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, a-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase. Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to a- glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms. -
Cellular localization
Lysosome. Lysosome membrane. -
Alternative names
- Acid Maltase
- Aglucosidase Alfa
- EC 3.2.1.20
see all
Images
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Alpha-Glucosidase Activity Assay Kit (ab174093)
Time course of Alpha-Glucosidase Activity in mouse liver tissue lysate
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Alpha-Glucosidase Activity Assay Kit (ab174093)Time course of Alpha-Glucosidase Activity in various samples
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Alpha-Glucosidase Activity Assay Kit (ab174093)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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a-Glucosidase kinetic assayKinetic profile of various amounts (0, 2, 4, 6, 8 & 10 mU) of α-glucosidase run at 25°C under this protocol. Inset: Results for 0-0.2-0.4-0.6-0.8-1.0 mU of α-glucosidase. Data points after 5 minutes were used to determine slope. This is example data only.