Antibodies, Proteins, Kits and Reagents for Life Science

Alexa Fluor® 594 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab203429)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Alexa Fluor® 594 Rabbit monoclonal [EPR4844] to SQSTM1 / p62 - Autophagosome Marker
Suitable for: ICC/IF
Knockout validated
Reacts with: Human
Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Product name

Alexa Fluor® 594 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker
See all SQSTM1 / p62 primary antibodies
Description

Alexa Fluor® 594 Rabbit monoclonal [EPR4844] to SQSTM1 / p62 - Autophagosome Marker
Host species

Rabbit
Conjugation

Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Tested Applications & Species

Application Species
ICC/IF
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: MCF7 cells, HeLa cells (untreated and chloroquine-treated), HAP1 cells (untreated and chloroquine-treated). HAP1-SQSTM1 knockout cells have been used as a negative cell line.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Alexa Fluor^® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor^® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor^® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor^® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Application	Species
ICC/IF	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4844
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab203429)

ab203429 staining SQSTM1 in wild-type HAP1 cells, untreated and chloroquine-treated (50μM, 24 hours) and chloroquine-treated SQSTM1 knockout HAP1 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203429 at 1/500 dilution (shown in pseudocolor red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab203429)

ab203429 staining SQSTM1/p62 in HeLa cells +/- Chloroquine (50μM, 24 hours). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203429 at 1/500 dilution (shown in pseudocolor) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab203429)

ab203429 staining SQSTM1 / p62 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203429 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min).
Alexa Fluor® 594 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab203429)

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