Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] (ab205812)
![Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] (ab205812)](https://www.abcam.com/ps/products/205/ab205812/Images/ab205812-319511-ICCAP254186401.jpg "Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] (ab205812)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [2G11] to M6PR (cation independent)
- Suitable for: ICC/IF
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG
Overview
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Product name
Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11]
See all M6PR (cation independent) primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [2G11] to M6PR (cation independent) -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Full length native protein (purified) corresponding to Cow M6PR (cation independent).
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Epitope
This antibody is shown to recognize an epitope in the extracellular domain of Mannose 6 Phosphate Receptor. -
Positive control
- ICC/IF: HeLa cells, HAP1-WT and HAP1-IGF2R knockout cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
2G11 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] (ab205812)
ab205812 staining M6PR in wild-type HAP1 cells (top panel) and IGF2R knockout HAP1 cells (bottom panel). The cells were fixed with 4% PFA for 10 min., permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab205812 at 5μg/ml and ab195889 (Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 594)) at 1/250 dilution (shown in pseudocolour red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] (ab205812)](https://www.abcam.com/ps/products/205/ab205812/Images/ab205812-250107-ICCIFAP25418641.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [2G11] (ab205812)ab205812 staining M6PR (cation independent) in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205812 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
