Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab275988)
![Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab275988)](https://www.abcam.com/ps/products/275/ab275988/Images/ab275988-453273-anti-cytokeratin-20-antibody-epr1622y-immunohistochemistry-human-colon.jpg "Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab275988)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR1622Y] to Cytokeratin 20
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [EPR1622Y]
See all Cytokeratin 20 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR1622Y] to Cytokeratin 20 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human colon tissue. ICC: HT-29 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1622Y -
Isotype
IgG2 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab275988)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Cytokeratin 20 with ab275988 at 1/200 (5.0 µg/ml) dilution (Green).
Positive staining on human colon.
The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) 110℃ for 20 mins. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab275988 at 1/200 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount ®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab275988)](https://www.abcam.com/ps/products/275/ab275988/Images/ab275988-453274-anti-cytokeratin-20-antibody-epr1622y-immunocytochemistry-ht29.jpg)
Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab275988)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labelling Cytokeratin 20 with ab275988 at 1/500 (1.0 µg/ml) dilution (Green).
Confocal image showing cytoplasmic staining in HT-29 cells.
ab195889 anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
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![Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab275988)](https://www.abcam.com/ps/products/275/ab275988/Images/ab275988-454114-benefitsofrecombinantantibodies.png)
Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [EPR1622Y] (ab275988)
