Aldosterone ELISA kit (ab136933)
Key features and details
- Sensitivity: 4.7 pg/ml
- Range: 3.9 pg/ml - 250 pg/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mouse, Rat, Human, Mammals
Overview
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Product name
Aldosterone ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Aldosterone 20 84.8pg/ml 4.5% Aldosterone 20 27.7pg/ml 4.4% Aldosterone 20 13.9pg/ml 6.6% Inter-assay Sample n Mean SD CV% Aldosterone 13 72.6pg/ml 10.8% Aldosterone 13 24.4pg/ml 18% Aldosterone 13 10.8pg/ml 16.3% -
Sample type
Urine, Serum, Plasma -
Assay type
Competitive -
Sensitivity
= 4.7 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine = 98.7 7.5pg/ml - 120pg/ml Serum = 69.8 7.5pg/ml - 120pg/ml Plasma = 108.3 7.5pg/ml - 120pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse, Rat, Human, Mammals -
Product overview
Abcam’s Aldosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Aldosterone in plasma, serum and urine.
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Aldosterone antigen and a polyclonal sheep antibody specific to Aldosterone. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Aldosterone captured in the plate.
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Notes
Compound % Cross Reactivity 11-Deoxycorticosterone
0.30%
Progesterone
0.20%
Corticosterone 0.19%
Cortisol ≤ 0.001%
DHT ≤ 0.001%
Estradiol ≤ 0.001%
Testosterone ≤ 0.001%
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Aldosterone Alkaline Phosphatase Conjugate 1 x 5ml Aldosterone Antibody 1 x 5ml Aldosterone Standard 1 x 250µl Assay Buffer 1 x 27ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
Images
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ab136933 Aldosterone ELISA kit
Aldosterone measured in various samples showing quantity (pg) per mL of sample tested.
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Typical Standard CurveRepresentative standard curve using ab136933.