Albumin Depletion Kit (ab241023)
Overview
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Product name
Albumin Depletion Kit
See all Albumin kits -
Product overview
Albumin Depletion Kit (ab241023) provides easy and user-friendly spin columns for the depletion/removal of abundant albumin protein from samples thus enhancing the sample analysis. The kit is based on the interaction of Cibacron Blue 3G-A (immobilized on beads) with albumin. Each spin column can bind ~2-3 mg of albumin and processes samples in less than one hour. After albumin depletion, samples can be used for subsequent ELISA, immunoassays and other downstream analyses such as gel electrophoresis and functional assays. These columns can also be used for purification and enrichment of a variety of Cibacron Blue dye-binding proteins
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 25 tests Albumin Binding Buffer 1 x 33ml Albumin Binding Spin Column 1 x 25 units Albumin Elution Buffer 1 x 25ml -
Research areas
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- alb
- ALBU_HUMAN
- Albumin (32 AA)
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Images
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Depletion of albumin from plasma using ab241023.
Samples were analyzed by 15% SDS-PAGE. M: Protein Marker, 1: Eluted albumin fraction (20 µL of concentrated eluted albumin, primarily containing albumin), 2: Albumin-depleted plasma sample (20 µL of combined and concentrated flow-through and wash fractions).