Albumin Assay Kit (Fluorometric) (ab241017)
Key features and details
- Detection method: Fluorescent
- Sample type: Saliva, Urine
Overview
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Product name
Albumin Assay Kit (Fluorometric)
See all Albumin kits -
Detection method
Fluorescent -
Sample type
Saliva, Urine -
Product overview
The Albumin Assay Kit (Fluorometric) (ab241017) provides a simple, sensitive, and high-throughput adaptable assay that detects albumin concentrations in healthy and Albuminuric urine samples.
This assay is based on the fluorometric detection of albumin using a probe (AB580) that specifically recognizes albumin (Ex/Em: 600/630 nm). AB580 does not cause fluorescence in the presence of other proteins or metabolites such as IgG, urea, ascorbic acid and glucose. Our assay can detect albumin concentrations as low as 20 mg/L.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Albumin Assay Buffer 1 x 7ml Albumin Diluent 1 x 7ml Albumin Probe (AB580) 1 x 0.4ml BSA Standard (2 mg/ml) 1 x 1ml -
Research areas
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all
Images
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BSA Standard Curve.BSA Standard Curve.
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Relative fluorescence of selected metabolites and proteins found in human urine.Human Serum Albumin (30 µg) was assayed and compared with 2) Ascorbic Acid (20 mM), 3) Glucose (20 mM), 4) Creatinine (20 mM), and 5) Urea (20 mM), 6) Lysozyme (30 µg), 7) Trypsin (30 µg), and 8) IgG (30 µg).
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Measurement of Albumin in urine from healthy and diabetic donors (40 µL each), and saliva (30 µL).Samples were assayed following kit protocol. Albumin concentrations (in mg/L) are: healthy donor urine: 19.4; diabetic donor urine: 49.0; Saliva: 290.
