Astana Biomed Group, an authorized Abcam distributor in Central Asia

AKT2 ELISA Kit (ab208986)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 5 pg/ml
  • Range: 78.13 pg/ml - 5000 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    AKT2 ELISA Kit
    See all AKT2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    NIH 3T3 8 3.6%
    Inter-assay
    Sample n Mean SD CV%
    NIH 3T3 3 7.3%

  • Sample type

    Cell culture supernatant, Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    5 pg/ml

  • Range

    78.13 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture media 105 103% - 107%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Product overview

    AKT2 ELISA Kit (ab208986) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AKT2 protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate AKT2 with 5 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases. These kinases regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Specifically, AKT2 seems to be the principal isoform responsible for the regulation of glucose uptake. The AKT kinases mediate these processes through serine and/or threonine phosphorylation of a range of downstream substrates.

    Human and rat AKT2 exhibit 98.1% and 98.5% amino acid identity to mouse AKT2, respectively.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - AKT2 ELISA Kit (ab208986)
    Other - AKT2 ELISA Kit (ab208986)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of AKT2 standard curve in Sample Diluent NS.
    Example of AKT2 standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of AKT2 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
    Example of AKT2 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spiked AKT2 in cell culture media.
    Interpolated concentrations of spiked AKT2 in cell culture media.

    The concentrations of AKT2 were measured in duplicates, interpolated from the AKT2 standard curve and corrected for sample dilution. Undiluted samples are as follows: cell culture 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AKT2 concentration was determined to be 1.3 ng/mL in RPMI base media.

  • Interpolated concentrations of native AKT2 in mouse NIH 3T3, mouse C2C12, human MCF7, and rat PC12 extracts based on 25 µg/mL, 50 µg/mL, 100 µg/mL, and 37.5 µg/mL extract loads, respectively.
    Interpolated concentrations of native AKT2 in mouse NIH 3T3, mouse C2C12, human MCF7, and rat PC12 extracts based on 25 µg/mL, 50 µg/mL, 100 µg/mL, and 37.5 µg/mL extract loads, respectively.

    The concentrations of AKT2 were measured in duplicate and interpolated from the AKT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AKT2 concentration was determined to be 0.74 ng/mL in NIH 3T3 extract, 1.45 ng/mL in C2C12 extract, 4.47 ng/mL in MCF7 extract, and 1.85 ng/mL in PC12 extract.

  • Sandwich ELISA - AKT2 ELISA Kit (ab208986)
    Sandwich ELISA - AKT2 ELISA Kit (ab208986)
    To learn more about the advantages of recombinant antibodies see here.

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