AGE (Advanced Glycation End) Assay Kit (ab238539)
Key features and details
- Assay type: Competitive
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Purified protein, Serum, Tissue Lysate
- Sensitivity: 0.5 µg/ml
Overview
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Product name
AGE (Advanced Glycation End) Assay Kit
See all AGE kits -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Cell Lysate, Purified protein, Tissue Lysate -
Assay type
Competitive -
Sensitivity
0.5 µg/ml -
Range
0.36 µg/ml - 100 µg/ml -
Product overview
AGE (Advanced Glycation End Products) Assay Kit (ab238539) is designed for the rapid detection and quantitation of advanced glycation end product protein adducts.
Advanced Glycation End Products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent advanced glycation end products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The quantity of AGE adduct in protein samples is determined by comparing its OD with that of a known AGE-BSA standard curve.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 100X Conjugate Diluent 1 x 300µl 10X Wash Buffer 1 x 100ml AGE Conjugate 1 x 50µl AGE-BSA Standard 1 x 125µl Anti-AGE Antibody (1000X) 1 x 10µl Assay Diluent 1 x 50ml Protein Binding Strip Well Plate 1 unit Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl Stop Solution 1 x 12ml Substrate Solution 1 x 12ml -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
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Relevance
The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure. -
Cellular localization
Cell Membrane and Secreted -
Alternative names
- Advanced glycation end products
- AGEs
Images
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Example AGE-BSA Competitive ELISA Standard Curve.
Typical AGE Competitive ELISA results.
This data is for reference only and should not be used to interpret actual results.
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Advanced Glycation End Product Formation Pathways.Advanced Glycation End Product Formation Pathways.
CML = N-epsilon-(carboxymethyl)lysine.
GOLD = glyoxal-derived lysine dimer.
CEL = N-epsilon-(1-carboxyethyl)lysine.
MOLD = methylglyoxal-derived lysine dimer.
DOLD = 3-deoxyglucosone-derived lysine dimer.
3-DG = 3-deoxyglucosone.