Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.2 nM
Overview
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Product name
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric)
See all Adipogenesis kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
> 0.2 nM -
Range
0.2 nM - 10 nM -
Assay time
0h 40m -
Product overview
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513) quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (ODmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 0.2 - 10 nmol of triglyceride in Visit our FAQs page for tips and troubleshooting.
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Notes
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Adipogenesis Assay Buffer WM 1 x 25ml Adipogenesis Enzyme Mix Green 1 vial Adipogenesis Probe (in DMSO solution) Red 1 x 200µl Lipase Orange 1 vial Lipid Extraction Solution NM 1 x 10ml Triglyceride Standard Yellow 1 x 0.3ml -
Research areas
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Relevance
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
Images
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Functional Studies - Adipogenesis Detection Kit (ab102513)
Example of a standard curve obtained with ab102513.
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Functional Studies - Adipogenesis Detection Kit (ab102513)Example of a standard curve obtained with ab102513.
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Functional Studies - Adipogenesis Detection Kit (ab102513) - FluorometricGlycerol measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
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Functional Studies - Adipogenesis Detection Kit (ab102513) - ColourimetricGlycerol measured colourimetrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample.
Ms brain value retrieved from 1:9 dilution of original 12.4mg/mL sample.
Ms muscle value retrieved from 1:9 dilution of original 7.5mg/mL sample.
Ms heart value retrieved from 1:27 dilution of original 16.4mg/mL sample.
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Functional Studies - Adipogenesis Detection Kit (ab102513) - ColourimetricGlycerol measured colourimetrically in cell lysates showing quantity (nmol) per 1 mln of tested cells
