Astana Biomed Group, an authorized Abcam distributor in Central Asia

Acetylated Histone H3 Antibody Sampler Panel (ab218055)

Overview

  • Product name

    Acetylated Histone H3 Antibody Sampler Panel

  • Product overview

    The Acetylated Histone H3 Antibody Sampler panel offers trial sizes of recombinant rabbit monoclonal antibodies against acetylation marks on Histone H3. Antibodies in this panel have been validated for use in ChIP and multiple applications. For more information on the individual antibodies in this panel, please view the individual abID listed on the component table below.

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex assays. Please refer to the ‘Associated products’ section below.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • ChIP - Anti-Histone H3 (acetyl K27) antibody [EP16602] (ab177178)
    ChIP - Anti-Histone H3 (acetyl K27) antibody [EP16602] (ab177178)

    Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab177178 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads as a control sample (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

  • ChIP - Anti-Histone H3 antibody [EPR16987] (ab176842)
    ChIP - Anti-Histone H3 antibody [EPR16987] (ab176842)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab176842 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • ChIP - Anti-Histone H3 (acetyl K4) antibody [EPR16596] (ab176799)
    ChIP - Anti-Histone H3 (acetyl K4) antibody [EPR16596] (ab176799)

    Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab176799 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

  • ChIP - Anti-Histone H3 (acetyl K23) antibody [EPR17712] (ab177275)
    ChIP - Anti-Histone H3 (acetyl K23) antibody [EPR17712] (ab177275)

    Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab177275 (blue), and 20µl of Anti Rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

  • Acetylated Histone H3 Antibody Sampler Panel (ab218055)
    Acetylated Histone H3 Antibody Sampler Panel (ab218055)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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