5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (ab270709)
Overview
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Product name
5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 -
Product overview
5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (ab270709) enables simple and rapid conjugation of oligonucleotide barcodes to antibodies to enable cell surface protein analysis in Multiomic Cytometry on 10x Genomics Chromium Controller. This kit is compatible with Chromium Single Cell Immune Profiling.
The kit includes 10 distinct oligonucleotide barcodes ready to conjugate to 10 μg of antibody each. The protocol involves only a few steps, requires minimal hands-on time and doesn’t require any expertise in antibody conjugation or oligonucleotide sourcing.
Please find antibody buffer considerations and compatibility in the protocol booklet. For incompatible buffers and low antibody concentrations, please see our antibody purification and concentration kits.
For conjugating antibodies with additional distinct oligonucleotide barcodes, please see 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (ab270703) and 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (ab270705). 100 μg conjugation kits are available on-demand for each of these 30 oligonucleotide barcodes. For conjugating more than 30 antibodies with distinct oligonucleotide barcodes, please contact us to discuss your requirements.
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Notes
For oligonucleotide barcode sequence information, please see appendix in the protocol booklet.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit ab274246 - Conjugate Resuspension Buffer 1 x 1500µl ab274106 - Modifier reagent 1 x 200µl ab274267 - Oligo 10X5021 1 x 10µg ab274268 - Oligo 10X5022 1 x 10µg ab274269 - Oligo 10X5023 1 x 10µg ab274270 - Oligo 10X5024 1 x 10µg ab274271 - Oligo 10X5025 1 x 10µg ab274272 - Oligo 10X5026 1 x 10µg ab274273 - Oligo 10X5027 1 x 10µg ab274274 - Oligo 10X5028 1 x 10µg ab274275 - Oligo 10X5029 1 x 10µg ab274276 - Oligo 10X5030 1 x 10µg ab274244 - Quencher N 1 x 200µl ab274245 - Reagent C 1 x 500µg ab274243 - Reagent S 1 x 1250µl
Images
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5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® is part of the 10x Genomics Compatible Products
5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® is compatible with Chromium Single Cell Immune Profiling from 10x Genomics.
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Cell surface protein analysis using 10 antibody conjugates generated with 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® on 10x Chromium ControllertSNE projections of ~5,000 peripheral blood mononuclear cells (PBMCs) output by Cell Ranger PBMCs were grouped by cell surface protein expression profiles from 10 antibody conjugates for cell surface protein markers generated with 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link®. The additional information from antibody UMI counts better can enrich phenotype information of many canonical immune cell types compared to gene expression data alone - naive and memory T cells, CD4+ and CD8+ T cells (populations denoted with asterisk*), for example.
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Antibody conjugate and oligonucleotide barcode format for Chromium Single Cell Immune Profiling from 10x Genomics5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® includes oligonucleotide barcodes compatible with Chromium Single Cell Immune Profiling from 10x Genomics, which are in the format:
5’ CGGAGATGTGTATAAGAGACAGNNNNNNN
NNNXXXXXXXXXXXXXXXNNNNNNNNNCCCAT
ATAAGA*A*A 3’.
X represents the nucleotides of the unique barcode.
N represents a randomly selected A, C, G, or T.
The symbol * indicates a phosphorothioated bond, this modification renders the internucleotide linkage resistant to nuclease degradation.