3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate
- Sample type: Adherent cells, Cell culture extracts, Suspension cells, Tissue Extracts
Overview
-
Product name
3' to 5' Exonuclease Activity Assay Kit (Fluorometric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269) provides a quick and easy method for monitoring 3’ exonuclease activities in a wide variety of samples. In this assay, exonuclease digests the provided DNA probe, producing a strong fluorescent signal (Ex/Em = 304/369nm). The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.2 µU of exonuclease activity.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Exonuclease Assay Buffer 1 x 25ml Exonuclease Positive Control (Lyophilized) 1 vial Exonuclease Probe (Lyophilized) 1 vial Fluorescence Standard (2 mM in DMSO) 1 x 100µl Half-area Plate 1 unit
Images
-
3' to 5' Exonuclease Kinetics
Kinetic curves of rat liver extract (40.6 μg), HeLa cell lysate(12.2 μg) and E.coli (0.642 μg).
-
3' to 5' Exonuclease Activity in different Sample typesSpecific activity from rat liver extract (0.0206 μU/μg). HeLa cell lysate (0.0841 μU/μg) and E.coli crude extract (0.9071 μU/μg). Assays performed according to the kit protocol.
-
3' to 5' Exonuclease ActivitySpecific activity of the probe to 3’ to 5’ Exonuclease (Exonucleae III) in contrast to DNAase I. Same units of proteins were added as defined by the commercially available values.
-
Fluorescence Standard CurveTypical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Fluorescence Standard Curve.
