Ustekinumab ELISA Kit (Stelara®) (ab264502)
Key features and details
- Sensitivity: 3 ng/ml
- Range: 3 ng/ml - 100 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Ustekinumab ELISA Kit (Stelara®) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
3 ng/ml -
Range
3 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Ustekinumab ELISA Kit (Stelara®) (ab264502) is the only FDA-approved medicine that targets IL-12 and IL-23, which are thought to be associated with gastrointestinal inflammation in Crohn's disease. This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of ustekinumab in the sample or standard. Results of samples can be determined directly using the standard curve.
Ustekinumab is a fully human monoclonal antibody that binds with high specificity and affinity to the cytokines interleukin (IL)-12 and IL-23, thereby suppressing IL-12- and IL-23-mediated inflammation associated with psoriasis.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Assay Buffer 2 x 50ml HRP-conjugate Probe 1 x 12ml Micro ELISA plate 1 unit Plate Sealer 2 units Stop Solution 1 x 12ml TMB Substrate 1 x 12ml Ustekinumab Standard S1 1 x 200µl Ustekinumab Standard S2 1 x 200µl Ustekinumab Standard S3 1 x 200µl Ustekinumab Standard S4 1 x 200µl Ustekinumab Standard S5 1 x 200µl Ustekinumab Standard S6 1 x 200µl Ustekinumab Standard S7 1 x 200µl Wash Buffer (20X) 1 x 50ml
Images
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Example Standard Curve
This standard curve is for demonstration only.
A standard curve must be run with each assay.