U-87 MG nuclear extract lysate (ab14903)
Overview
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Product name
U-87 MG nuclear extract lysate -
General notes
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading... -
Research areas
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Background
Glioblastoma is the highest dedifferentiated form of astrocytic brain tumours, and it is refractory to chemotherapy in most cases. U-87 MG (malignant glioma) cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells. The lysate is a good positive control for prostate apoptosis response-4 (Par-4) which is upregulated in prostate tumor cells undergoing apoptosis (several Abcam Par-4 abs). It is also a good positive control for Alpha-1-ACT, a serine protease inhibitor tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain.