Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Plasma, Serum, Suspension cells, Tissue
- Sensitivity: 40 mU/ml
Overview
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Product name
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity -
Sensitivity
> 40 mU/ml -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples. In this kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the enzyme mix and developer to form a colored product with strong absorbance at OD=450 nm.
The assay is simple, sensitive and high-throughput and can detect TPI activity as low as 40 mU/mL.
TPI assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 20-40 min -
Notes
Triose Phosphate Isomerase (TPI, TIM, EC 5.3.1.1) is an important enzyme for glycolysis. It catalyzes the reversible conversion of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms.
In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests NADH Standard Yellow 1 vial Positive Control 1 vial TPI Assay Buffer 1 x 25ml TPI Developer 1 vial TPI Enzyme Mix 1 vial TPI Substrate 1 vial -
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. -
Involvement in disease
Triosephosphate isomerase deficiency -
Sequence similarities
Belongs to the triosephosphate isomerase family. -
Post-translational
modificationsThe initiator methionine for isoform 2 is removed. - Information by UniProt
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Alternative names
- TIM
- TPI1
- TPIS_HUMAN
see all
Images
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Functional Studies - Triosephosphate Isomerase Activity Assay Kit (Colorimetric) (ab197001)
Relative TPI activity was calculated in lysates prepared from rat muscle (0.5 µg), MCF-7 cells (0.8 µg), and HeLa cells (0.84 µg).
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Functional Studies - Triosephosphate Isomerase Activity Assay Kit (Colorimetric) (ab197001)Typical NADH Standard Curve obtained following assay protocol.
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Functional Studies - Triosephosphate Isomerase Activity Assay Kit (Colorimetric) (ab197001)Kinetic measurement of Triose Phosphate Isomerase (TPI) activity in various samples.