Tissue Clearing Reagent 1 and 2 combo (ab243316)
Overview
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Product name
Tissue Clearing Reagent 1 and 2 combo
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Product overview
Tissue Clearing Reagent 1 and 2 combo, ab243316, contains both clearing reagents, which are components of the complete kit: Tissue Clearing Kit ab243298. The complete kit enables the easy set-up of rapid tissue clearing for 3D tissue imaging using simple protocols and standard laboratory equipment.
Please see ab243298 for more information and the protocol.
The kit enables you to set up tissue clearing easily and clear tissue quickly. The protocols are simple and use standard laboratory equipment, and can be used with immunostaining, fluorescent proteins and chemical dyes. Clearing is reversible so that you can treat your sample for conventional H&E or IHC staining after 3D imaging.
To help you get started with tissue clearing, we have tissue clearing validated antibodies. Validated antibodies are for key markers, such as NeuN, GFAP, Iba1, etc, and are tested for tissue clearing with 1 mm mouse brain sections.If you want to set up tissue clearing on a sample type that we haven’t tested or to use your own antibodies, please consult the protocol book for this kit.
To learn more about tissue clearing, and tissue clearing methods, please review our guide to tissue clearing.
Tissue clearing reagents and companion products are also available as individual products.
These tissue clearing reagents were developed with Visikol Inc, who developed the Visikol® HISTO™ tissue clearing technology which is the basis of our reagents.
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Tested applications
Suitable for: Histologymore details
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 10 ml 30 ml 100 ml Tissue Clearing Reagent 1 1 x 10ml 1 x 30ml 1 x 100ml Tissue Clearing Reagent 2 1 x 10ml 1 x 30ml 1 x 100ml -
Relevance
The goal for tissue clearing is making large, fixed biological samples transparent. “Large” in this case means thick sections of tissue, whole organoids, entire organs or even entire young rats, ranging in thickness from around 100 µm to several centimeters. Typically, samples of this size are not transparent, and are therefore difficult to analyze using visible wavelengths of light with a microscope. Tissue clearing involves a series of chemical steps that render a large sample transparent.
