Threonine Assay Kit (ab239726)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Threonine Assay Kit -
Detection method
Fluorescent -
Product overview
The Threonine Assay Kit (ab239726) allows for highly sensitive quantification of L-Threonine levels in biological fluids and tissues. The assay is based on the selective, NAD+-coupled enzymatic metabolism of threonine, yielding an oxidized intermediate and NADH. A developer enzyme mixture utilizes the NADH generated to convert the probe into a stable fluorophore (Ex/Em = 535/587 nm). The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect threonine levels down to 2 μM in samples.
Sample Type:
- Human or animal biological fluids (plasma, serum, CSF, etc.)
- Soft tissue homogenates (i.e. liver, brain, etc.)
- Cultured cell lysates (adherent or suspension cells) or cell culture growth medium
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer Enzyme Mix 1 vial L-Threonine Standard 1 vial Probe solution 1 x 200µl Threonine Assay Buffer 1 x 25ml Threonine Enzyme Mix 1 vial -
Research areas
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Relevance
Threonine is one of the 20 natural amino acids. Nutritionally, in humans, threonine is an essential amino acid.
Images
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Threonine Standard Curve.
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Specificity for detection of L-Threonine (L-THR) over D-Threonine and other common amino acids.At a 10-fold molar excess (5 nmole/well) versus L-Threonine (500 pmole/well), all other amino acids tested contribute ≤5% interference.
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Estimation of total L-Threonine in pooled normal human plasma (10 µl), single donor off-the-clot human serum (5 µl) and pooled human CSF (10 µl).L-Threonine concentrations for plasma, serum and CSF samples were 93.26 ± 3.72 μM, 159.7 ± 8.36 μM and 70.92 ± 3.12 μM, respectively. Data are mean ± SEM of at least 3 replicates, samples were deproteinized using 10 kDa MWCO spin columns and assayed according to the kit protocol.