THP-1 nuclear extract lysate (PMA stimulated) (ab14901)
Overview
-
Product name
THP-1 nuclear extract lysate (PMA stimulated)
See all THP1 lysates -
Description
THP-1 nuclear extract lysate -
General notes
Cell line: THP1 (Human acute monocytic leukemia).
The protein content has been determined by a Bradford-based assay.
The THP-1 nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with or without PMA (10 ng/ml).
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.0002% Sodium molybdate, 0.216% Beta glycerophosphate, 0.018% Sodium orthovanadate, 0.042% Sodium fluoride, 0.015% DTT, 0.48% HEPES, 0.003% EDTA, 20% Glycerol, 2.34% Sodium chloride
With Protease inhibitors. -
Concentration information loading... -
Research areas
-
Background
Properties: differentiation; lysozyme synthesis; phagocytosis; Fc receptor; IL-1 prod.; C3b receptor. The cell line can differentiate into macrophage-like cells (phagocytic) and can be used for induction of differentiation studies. When stimulated with PMA cells have been reported to differentialte into a monocytic pathway and release arachidonic acid and prostanoids.
Images
-
Functional Studies - THP1 nuclear extract lysate (PMA stimulated) (ab14901)
Each lot has been tested for PPAR-gamma activation by using TransAM™ PPAR-gamma Kits. The signal intensity for PPAR-gamma activation in each lot is compared to the signal intensity obtained with extracts from unstimulated THP-1 cells (see figure). After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of PPAR-gamma activation in a given cell type.