Testosterone ELISA Kit (ab178655)
Key features and details
- Sensitivity: 2.96 pg/ml
- Range: 10 pg/ml - 210 pg/ml
- Sample type: Saliva
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Testosterone ELISA Kit
See all Testosterone kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Saliva 16 Inter-assay Sample n Mean SD CV% Saliva 12 -
Sample type
Saliva -
Assay type
Competitive -
Sensitivity
>= 2.96 pg/ml -
Range
10 pg/ml - 210 pg/ml -
Recovery
98.97 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in saliva.
A 96-well plate has been precoated with anti-Testosterone antibodies (solid-phase). Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Testosterone IgG Coated Microplate (12 x 8 wells) 1 unit Control A 1 x 1ml Control B 1 x 1ml Cover Foil 1 unit Incubation Buffer 1 x 30ml Stop Solution 1 x 15ml Strip holder 1 unit Testosterone HRP Conjugate 1 x 1ml Testosterone Standard 0 - 0 pg/mL 1 x 1ml Testosterone Standard 1 - 10 pg/mL 1 x 1ml Testosterone Standard 2 - 50 pg/mL 1 x 1ml Testosterone Standard 3 - 200 pg/mL 1 x 1ml Testosterone Standard 4 - 1,000 pg/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
Research areas
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Alternative names
- 17ß-hydroxyandrost-4-ene-3-one
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Database links
- SwissProt: P04278 Human
Images
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Typical Standard Curve
Representative standard curve using ab178655.
