T47D nuclear extract lysate (ab14896)
Overview
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Product name
T47D nuclear extract lysate
See all T47D lysates -
General notes
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading... -
Research areas
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Background
T47D cells carry receptors for a variety of steroids and calcitonin. They express mutant tumor suppressor protein p53 protein. Under normal culturing conditions, they express progesterone receptor constitutively and are responsive to estrogen. They are able to lose the estrogen receptor (ER) during long-term estrogen deprivation in vitro. Cells are therefore used as a model for studies of drug resistance to tamoxifen in patients with mutant p53 breast tumors.