Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Overview
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Product name
Superoxide Dismutase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Enzyme activity -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Superoxide Dismutase Activity Assay Kit (Colorimetric) ab65354 is a simple and rapid assay for superoxide dismutase (SOD) activity.
In the SOD assay protocol:
- superoxide anions are produced by the action of xanthine oxidase
- SOD catalyzes the dismutation of the superoxide anion into hydrogen peroxide and O2
- superoxide anions act on WST-1 to produce a water-soluble formazan dye which can be detected by the increase in absorbance at 450 nm
The greater the activity of SOD in the sample, the less formazan dye is produced.Superoxide dismutase assay protocol summary:
- add samples to wells
- add WST-1 working solution and enzyme working solution and incubate for 20 min at 37ºC
- analyze with microplate reader -
Notes
Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.
Related products
Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 100 tests SOD Assay Buffer WM 1 x 20ml SOD Dilution Buffer NM 1 x 10ml SOD Enzyme Solution Green 1 x 20µl WST Solution Red 1 x 1ml -
Research areas
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Relevance
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). -
Cellular localization
Cytoplasmic -
Alternative names
- ALS
- ALS1
- Cu /Zn superoxide dismutase
see all
Images
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Functional studies - ab65354 Image from Park J et al., PLoS One 12(2), fig 4b. Doi: 10.1371/journal.pone.0172751 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Park J et al investigates the recovery in erectile function after administration of chronic statin alone in DM (streptozotocin (STZ)-induced diabetes mellitus) rats. SOD activity was determined using Superoxide Dismutase activity assay kit (ab65354).
* Indicates statistical significance in comparison with DM group (P # Indicates statistical significance in comparison with the statin group (P
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Functional Studies- Superoxide Dismutase Activity Assay Kit (ab65354)Superoxide dismutase measured in biofluids at various dilutions
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Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)Superoxidase dismutase (ab90040) measured showing inhibition rate (%) per concentration (microgram per mL)
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Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)Principle of Superoxide Dismutase Assay.
