Src Kinase Inhibitor I (SKI-1) (ab120839)
Key features and details
- Potent, selective and dual site Src tyrosine kinase family inhibitor
- CAS Number: 179248-59-0
- Purity: > 98%
- Soluble in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Src Kinase Inhibitor I (SKI-1) -
Description
Potent, selective and dual site Src tyrosine kinase family inhibitor -
Alternative names
- 4-(4-Phenoxyanilino)-6,7-dimethoxyquinazoline
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Biological description
Potent, selective and dual site Src family tyrosine kinase inhibitor (IC50 values are 44 and 88 nM for Src and Lck, respectively). Cell-permeable, reversible and ATP-competitive. Interacts with both ATP and peptide-binding sites. Additionally inhibits VEGFR2 and C-fms (IC50 values are 0.32 and 30 µM, respectively). Induces apoptosis.
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Purity
> 98% -
CAS Number
179248-59-0 -
Chemical structure
Properties
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Chemical name
6,7-Dimethoxy-N-(4-phenoxyphenyl)-4-quinazolinamine -
Molecular weight
373.41 -
Molecular formula
C22H19N3O3 -
PubChem identifier
1474853 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 50 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
COc1cc4c(cc1OC)ncnc4Nc3ccc(Oc2ccccc2)cc3 -
Source
Synthetic
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Research areas
Images
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Functional Studies - Src Kinase Inhibitor I (SKI-1) (ab120839)ab11512 staining E cadherin in SW480 cells treated with Src Kinase Inhibitor I (SKI-1) (ab120839), by ICC/IF. Increase of E cadherin expression correlates with increased concentration of Src Kinase Inhibitor I (SKI-1), as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120839 (Src Kinase Inhibitor I (SKI-1)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
