Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric) (ab252902)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Adherent cells, Tissue, Tissue Homogenate, Tissue Lysate
- Sensitivity: 50 µU
Overview
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Product name
Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric)
See all Sorbitol Dehydrogenase kits -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Tissue Homogenate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
50 µU -
Product overview
The Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric)(ab252902) provides a quick and easy way for monitoring SDH activity in various samples. In this Assay, Sorbitol Dehydrogenase utilizes a provided substrate while reducing NAD+ to form NADH. NADH reacts with the developer, leading to the formation of a chromophore with strong absorbance at OD 450 nm. The assay is simple, sensitive and can detect Sorbitol Dehydrogenase Activity less than 50 μU in variety of samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests NADH Standard 1 vial SDH Assay Buffer 1 x 25ml SDH Developer 1 vial SDH Positive Control 1 vial SDH Substrate 1 x 200µl -
Research areas
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Function
Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. -
Tissue specificity
Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level). -
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. -
Cellular localization
Mitochondrion membrane. Cell projection > cilium > flagellum. Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface. - Information by UniProt
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Alternative names
- DHSO_HUMAN
- L iditol 2 dehydrogenase
- L-iditol 2-dehydrogenase
see all
Images
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NADH standard curve.
NADH standard curve.
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Kinetic measurement of Sorbitol Dehydrogenase activity from various samples.Kinetic measurement of Sorbitol Dehydrogenase activity from various samples.
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Sorbitol Dehydrogenase activity in various tissues/cellsRelative SDH Activity was calculated in lysates (μg protein) prepared from Rat Liver (8 μg), Rat Kidney (10 μg), HeLa (42 μg) and Jurkat (73 μg).