Abcam’s SIRT5 Activity Assay Kit (Fluorometric) (ab210074) detects the deacetylase activity of recombinant or purified SIRT5. The kit is designed for the rapid and sensitive evaluation of SIRT5 inhibitors or activators using recombinant or purified SIRT5. 

The Abcam SIRT5 Deacetylase Fluorometric Assay Kit is a simple and homogeneous assay system designed to measure the deacetylase activity of SIRT5. This kit offers a unique method for assaying protein deacetylases that is a highly sensitive and convenient alternative to conventional radiolabelling or HPLC methods. The kit uses an acetylated peptide substrate that has a fluorophore and a quencher at its amino and carboxyl terminals. Once the substrate is deacetylated by SIRT5, it can be cleaved by the peptidase, releasing the fluorophore from the quencher. The subsequent increase in fluorescence intensity of the fluorophore is in direct proportion to the deacetylase activity of SIRT5.

This kit comes with Recombinant SIRT5, the acetylated peptide substrate, NAD as a co-substrate and other reagents necessary for the fluorescent deacetylase assay, all of which are ready-to-use.