RFC2 overexpression 293T lysate (whole cell) (ab94285)
Overview
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Product name
RFC2 overexpression 293T lysate (whole cell)
See all RFC2 lysates -
General notes
ab94285 is a 293T cell transfected lysate in which Human RFC2 has been transiently over-expressed using a pCMV-RFC2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP. Disease: Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Similarity: Belongs to the activator 1 small subunits family.
Images
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SDS-PAGE - RFC2 overexpression 293T lysate (whole cell) (ab94285)ab94285 at 15µg/lane on an SDS-PAGE gel.
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Western blot - RFC2 overexpression 293T lysate (whole cell) (ab94285)All lanes : Anti-RFC2 antibody (ab88502) at 1/500 dilution
Lane 1 :RFC2 overexpression 293T lysate (whole cell) (ab94285)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated, at 1/2500 dilution
