Recombinant woodchuck CD80 protein (Active) (Biotin) (ab271373)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant woodchuck CD80 protein (Active) (Biotin)
See all CD80 proteins and peptides -
Biological activity
Active in a woodchuck CTLA4:CD80 interaction assay.
Buffer is PBS with 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. The coat protein, woodchuck CTLA4 (wCTLA4), was added ON at 4 deg (various concentrations as indicated). Binding reaction initiated with addition of woodchuck B7-1-biotin (wB7-1-biotin) for 2h at RT. Binding was detected using Strep-HRP generated luminescence.
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Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Woodchuck -
Predicted molecular weight
52 kDa including tags -
Molecular weight information
Protein runs at higher MW on SDS-PAGE due to glycosylation. -
Amino acids
30 to 237 -
Tags
Avi tag C-Terminus , Fc tag C-Terminus -
Additional sequence information
C-terminal Avi-Tag fused to the Fc-region of Human IgG1.
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Conjugation
Biotin
Preparation and Storage
- Activation B7-1 antigen
- B lymphocyte activation antigen B7
- B7
Images
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Functional Studies - Recombinant woodchuck CD80 protein (Active) (Biotin) (ab271373)
Functional analysis of ab271373 in a woodchuck CTLA4:CD80 interaction assay.
Note: B7-1 = CD80.
Buffer is PBS with 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. The coat protein, woodchuck CTLA4 (wCTLA4), was added ON at 4 deg (various concentrations as indicated). Binding reaction initiated with addition of woodchuck B7-1-biotin (wB7-1-biotin) for 2h at RT. Binding was detected using Strep-HRP generated luminescence.
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SDS-PAGE - Recombinant Woodchuck CD80 protein (Tagged) (Biotin) (ab271373)SDS-PAGE analysis of ab271373.