Antibodies, Proteins, Kits and Reagents for Life Science

Recombinant staphylococcus aureus hlgC protein (Active) (ab233606)

Key features and details

Expression system: Escherichia coli
Active: Yes
Suitable for: Functional Studies, WB, SDS-PAGE

Product name

Recombinant staphylococcus aureus hlgC protein (Active)
Biological activity

Cytotoxicity can be detected in human neutrophils when used in combination with hIg B in a concentration range of 0.03-200 nM.
Expression system

Escherichia coli
Accession

Q07227
Protein length

Full length protein
Animal free

No
Nature

Recombinant
- Species
  
  Staphylococcus aureus
- Sequence
  
  ANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALIL KMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYVSLINYLPKN KIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQ QNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSEL PPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGN SYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN
- Predicted molecular weight
  
  33 kDa
- Amino acids
  
  30 to 315
- Additional sequence information
  
  This product is the mature full length protein from aa 32 to 315. The signal peptide is not included.

Functional Studies - Recombinant staphylococcus aureus hlgC protein (Active) (ab233606)

HL-60 (Human promyelocytic leukemia cell line) was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of hlg B tag-free and ab233606 at equimolar concentration for 3 hours at 37°C with 5% CO₂ and 95% humidity. Cellular viability was determined by adding XTT and incubation for additional 16 hours. OD’s were determined in the supernatants at 470/690 nm. Red squares represent (ab233606) and blue squares represent hIgC his-tag. EC₅₀ was found to be 2.84 nM for ab233606 and 5.53 nM for hIgC his-tag.
Western blot - Recombinant staphylococcus aureus hlgC protein (Active) (ab233606)

All lanes : Polyclonal hIgC antibody at 0.5 µg/ml

Lane 1 : Recombinant staphylococcus aureus hlgC protein (Active) (ab233606) at 0.1 µg
Lane 2 : Recombinant staphylococcus aureus hlgC protein (Active) (ab233606) at 0.05 µg
Lane 3 : Recombinant staphylococcus aureus hlgC protein (Active) (ab233606) at 0.025 µg

Secondary
All lanes : Anti-rabbit IgG-horseradish peroxidase (HRP) conjugate

TMB substrate
SDS-PAGE - Recombinant staphylococcus aureus hlgC protein (Active) (ab233606)

SDS-PAGE analysis of ab233606 under denaturing and reducing conditions.

Lane 1: 1 µg

Lane 2: 5 µg

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