Recombinant Rubella Virus spike glycoprotein E1 protein (Fc Chimera) (ab256429)
Key features and details
- Expression system: HEK 293 cells
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant Rubella Virus spike glycoprotein E1 protein (Fc Chimera)
See all Rubella virus E1 proteins and peptides -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Rubella virus -
Sequence
EEAFTYLCTAPGCATQAPVPVRLAGVRFESKIVDGGCFAPWDLEATGACI CEIPTDVSCEGLGAWVPAAPCARIWNGTQRACTFWAVNAYSSGGYAQLAS YFNPGGSYYKQYHPTACEVEPAFGHSDAACWGFPTDTVMSVFALASYVQH PHKTVRVKFHTETRTVWQLSVAGVSCNVTTEHPFCNTPHGQLEVQVPPDP GDLVEYIMNYTGNQQSRWGLGSPNCHGPDWASPVCQRHSPDCSRLVGATP ERPRLRLVDADDPLLRTAPGPGEVWVTPVIGSQARKCGLHIRAGPYGHAT VEMPEWIHAHTTSDPWHPPGPLGLKFKTVRPVALPRTLAPPRNVRVTGCY QCGTPALVEGLAPGGGNCHLTVNGEDLGAVPPGKFVTAALLNTPPPYQVS CGGESDRATARVIDPAAQSFTGVVYGTHTTAVSETRQTWAEWA -
Amino acids
583 to 1025 -
Tags
Fc tag C-Terminus -
Additional sequence information
16 amino acid Gly-Ser linker connecting protein to sheep IgG Fc (CH2-CH3). NP_062884.1
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Description
Recombinant Rubella virus E1 protein (Fc Chimera)
Preparation and Storage
- E1
- E1 envelope glycoprotein
- Glycoprotein E1
- Spike glycoprotein E1
Images
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SDS-PAGE - Recombinant Rubella virus E1 protein (ab256429)
SDS-PAGE analysis of 3.2 μg (Lane 1) and 1.7 μg (Lane 2) of ab256429 under reducing conditions.
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Functional Studies - Recombinant Rubella virus E1 protein (ab256429)Detection of anti-Rubella IgG in human serum.
Plate coated with 50 ng/well of antigens. E1 = ab256429
Antigens coated in bicarbonate-carbonate buffer pH 9.6 for 1 hour at RT. Blocked with 2% BSA/PBS for 2 hours at RT.
Washed x3 with Tris washing buffer.
Serum samples (Public Health England) diluted 1/201 in 1% BSA in PBS-T.
Secondary antibody was anti-Human-IgG-HRP diluted 1/10000 in 1% BSA in PBS-T. TMB detection.
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Functional Studies - Recombinant Rubella virus E1 protein (ab256429)Detection of anti-Rubella IgM in human serum.
Plate coated with 100 ng/well of antigens. E1 = ab256429
Washed x3 with Tris washing buffer.
Serum samples (Public Health England) diluted 1/201 in 1% BSA in PBS-T + 4% IgG/RF stripper. After standing for 30 minutes the diluted samples were centrifuged at 17,000 x g for 1 minute and the supernatant used for ELISA.
Secondary antibody was anti-Human-IgM-HRP diluted 1/10000 in 1% BSA in PBS-T. TMB detection.