Recombinant mouse Thrombopoietin protein (Active) (ab256114)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Alternative names
- C mpl ligand
- C-mpl ligand
- Megakaryocyte colony stimulating factor
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Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. -
Involvement in disease
Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. -
Sequence similarities
Belongs to the EPO/TPO family. -
Domain
Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant mouse Thrombopoietin protein (Active) (ab256114)
Functional analysis of ab256114.
MO7e cell proliferation: ≤5 ng/mL; ≥ 2.0 x 10E5 units/mg (typcial ED50 is
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SDS-PAGE - Recombinant mouse Thrombopoietin protein (Active) (ab256114)SDS-PAGE analysis of ab256114 (1μg) under reducing (Lane 1) and non-reducing (Lane 2) conditions. ab256114 is a monomer predicted to have a MW of 18.7 kDa.
4-20% Tris-Glycine gel. Coomassie Blue staining.
